Визначення віруліцидних властивостей нового біоциду “ДезCан”

The high concentration of farm birds in a limited area creates favorable conditions for the spread of viral diseases. In this regard, the veterinary service is faced with the task of preventing the outbreak of infectious diseases of the poultry and the further spread of the disease. For this purpose a complex of antiepizootic measures is carried out, the key point of which is the disinfection of poultry buildings, equipment, containers, inventory, etc. Industrial poultry is constantly in need of the latest modern disinfectants, which will have the following properties: high biocidal capacity, prolonged action, reduced aggressiveness to treated surfaces, safety for personnel and the environment, ease of use, moderate price. Over time, microorganisms can create resistance to disinfectants. In order to prevent this phenomenon, a permanent rotation of disinfestations at a poultry enterprise, which will be created on the basis of various active substances, is necessary. The goal of the work. Investigate the virilidine action of the new disinfectant “DezSan” for RNA and DNA-containing viruses. Materials and methods of research. To verify the virilidic effect, two series of experiments were carried out using the ducts of hepatitis RNA and the DNA-virus of smallpox of the pox. To determine the effectiveness of the virilidic concentration of “DezSan” in relation to the bird pox virus – DNA-content was cultured on chorionic-alantium shell in the 10–12 day chick embryos and to the hepatitis virus duckling virus-RNA-purposive, a suspension of the virulent material that was obtained after propagation virus in primary cultures of dactylon embryo cells. For the experiment, 10 mattresses were used. The virus content of the liquid was mixed with equal volume of disinfectant solution “DezSan”, withstand 15, 30 and 60 minutes. In this case, used 0.1%, 0.25%, 0.5% and 1% solution of disinfectant “DezSan”. The effectiveness of decontamination of test object surfaces from the pox virus and the hepatitis virus ducts disinfectant was checked in the following order: on a sterilized surface of test objects, a sterile pipette was applied to a suspension of 1 to 2 cm3 of the virus. Contaminated test objects were left in the cuvettes horizontally and vertically, dried for 1–2 hours and with the help of the sprayer, the wetted surface was treated with an experimental disinfectant solution, taking into account the concentration, exposure and amount of disinfectant used. Results of research and discussion. “DezSan” in a concentration of 0.1% after 15 minutes. Inactivated hepatitis virus ducts by 46.3%; after 30 minutes – by 91.1%, and in an hour – by 94.1%. At 0.25% concentration of the solution at exposure for 15 minutes. the death of the virus was observed at 99.5%. 30 minutes later and 1 hour – the virus of hepatitis ducklings has been neutralized by 100%. Complete inactivation of the virus in 15 minutes was carried out in the treatment of test surfaces of 0.5% and 1% solution “DezSan”. In the washings taken after 30 and 60 minutes from surfaces treated with 0.5% and 1.0% solution of disinfectant, changes in test systems were not detected. 0.1% solution of disinfectant “DezSan” after 15 minutes. inactivated viral particles by only 45.7%. 30 minutes later the virus was already eradicated by 93.0%, and after 1 hour – by 96.0%. A solution of “DezSan” at a concentration of 0.25% after 15 min. destroying 99.6% of viral particles. And after 30 minutes and 1 year completely inactivated the virus. Thus, the experimental means “DezSan” in the concentration of 0.5% and 1.0% showed a pronounced virilidine activity and is capable of within 30 and 60 minutes. completely destroy the virus of hepatitis Ducklings. At 0.25% of the solution “DezSan” after 15 minutes. 99.7% of the viral parasites of the pox had been disposed of. And after 30 minutes and for 1 hour, the vehicle at the same concentration completely inactivated the virus. It should be noted that in the concentration of 0.5% and 1.0%, “DezSan” had a pronounced virilidine activity and could completely inactivate the pox virus pox during all controlled periods. Conclusions and prospects for further research. It has been proved that the “DezSan” biocide has expressed virilidic properties in relation to the RNA-containing virus of infectious hepatitis ducts during exposure for 15 minutes at concentrations of 0.25% and above. It was found that 0.25% solution of disinfectant “Dezsan” at exposures of 30 and more minutes completely kills the DNA – containing pox virus of smallpox. The drug “DezSan” can be used for disinfection measures in poultry farms with viral diseases, as it is effective against RNA and DNA-containing viruses. In future, the sporocidal properties of the biocide “DezSan” will be studied.В статті наведенні дані дослідження віруліцидних властивостей нового вітчизняного біоциду “ДезCан”. Промислове птахівництво постійно потребує новітніх сучасних дезінфекційних засобів, які будуть володіти такими властивостями: висока біоцидна здатність, пролонгована дія, знижена агресивність до поверхонь, що оброблюються, безпечність для персоналу та навколишнього середовища, простота застосування, помірна ціна. З часом у мікроорганізмів можливе утворення резистентності до дезінфікуючих засобів. Щоб запобігти даному явищу необхідна постійна ротація деззасобів на птахівничому підприємстві, що будуть створені на основі різних діючих речовин. Дослідження проводили в умовах лабораторії “Ветеринарна фармація” Сумського національного аграрного університету та лабораторії Сумської Державної біологічної фабрики. Для перевірки віруліцидної дії були проведені дві серії дослідів з використанням РНК-вірусу гепатиту каченят та ДНК-вірусу віспи птиці. Визначення ефективності знищення вірусу віспи птиці дезінфектантом проводили методом знезараження тест-об’єктів і суспензійним методом. Доведено, що біоцид “ДезCан” має виражені віруліцидні властивості щодо РНК-вмістного вірусу інфекційного гепатиту каченят при експозиції 15 хвилин у концентраціях 0,25% та вище. Встановлено, що 0,25% розчин дезінфектанту “ДезCан” за експозиції 30 та більше хвилин повністю вбиває ДНК-вмісний вірус віспи птиці. Біоцид “ДезCан” може бути використаний при проведенні дезінфекційних заходів в птахівничих господарствах при вірусних захворюваннях, оскільки він ефективний щодо РНК та ДНК-вмісних вірусів